World Journal of Pathology Volume No 6

Original Research Open Access

Multiple human papillomavirus infection in ASC-H and HSIL lesions in women with cervical biopsies and comparison between Cobas 4800 HPV and Hybrid capture High-risk 2 Test.

Catteau Xavier, Petein Michel, Vanhaeverbeek Michel and Noël Jean-Christophe
World Journal of Pathology 2012, 1:15



The primary aim of this study was to document the distribution of different HPV genotypes in women with cytological lesions of ASC-H and HSIL in whom a biopsy was performed. The secondary aim was to evaluate the performance of the hc2 test by comparing the results with those obtained with the Cobas 4800 test.


A total of 84 liquid-based cytology, 34 ASC-H and 50 HSIL, were collected for this study. Biopsy specimens of lesions were obtained in 68 cases. Cytlogical samples were tested with Hybrid Capture 2 and Cobas 4800.


Eleven had no dysplasia, seven had CIN 1, twelve had CIN 2, eight had CIN2/3, twenty-six had CIN3, and four had SCC on biopsy. There was no statistically significant difference between age groups or histological lesions regarding the presence of co-infection. The detection rate of HR-HPV DNA was slightly higher using hc2 compared with Cobas 4800. Indeed, one specimen (lesion of ASC-H with CIN3 on biopsy) was hc2 positive, while Cobas 4800 negative. However, the results suggest that the clinical performances of both methods can be compatible.


There was no statistically significant difference in the presence of co-infection depending on the grade of dysplasia, nor according to age. The advantage of the Cobas 4800 is the ability to subtype HPV but at present it is not essential for the care of patients. In the case of our laboratory where hc2 is used, at present time there appears to be no particular reason to change the tests for HPV detection.

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