World Journal of Pathology Volume No 6

Research Open Access

Masson’s Trichrome and AgNOR Study of Fibrous Dysplasia and Ossifying Fibroma in Lagos University Teaching Hospital Patients

Olujide Soyele, Olajumoke Effiom and Onatolu Odukoya
World Journal of Pathology 2013, 2:16

Abstract

Introduction

Fibrous dysplasia (FD) and ossifying fibroma(OF) pose diagnostic challenges.This study attempts to differentiate between the two lesions, using AgNOR and Masson’s Trichrome staining techniques.

Study design

A retrospective study on FD and OF.

Materials and methods

Forty cases each of fibrous dysplasia and ossifying fibroma were randomly selected from the oral biopsy archives of Lagos University Teaching Hospital. Three 5 microns paraffin sections per case were stained with Hematoxylin and Eosin, Masson’s Trichrome and AgNOR respectively.   Proportions of bone trabeculae type and mean AgNOR scores for both lesions were analyzed for χ² and t statistics respectively using the Epi-info statistical package.

Results

Proportions of Lamella bone trabeculae type in ossifying fibroma(52.5%) was significantly higher than in fibrous dysplasia (30.0%);  proportions of  mixed bone trabeculae type  in fibrous dysplasia (70.0%) was significantly higher than in ossifying fibroma (50.0%) (p< 0.05).  Proportions of woven bone trabeculae type   in fibrous dysplasia (60.0%) was not significantly higher than in ossifying fibroma (55.0%) (p> 0.05). Mean AgNOR score for ossifying fibroma (130.4 ± 32.3/ 100 cells) was significantly higher than for fibrous dysplasia (92.3 ± 27.4/ 100 cells) (p < 0.05).

Conclusion

Proportions of Lamella and mixed bone trabeculae types and mean AgNOR scores are important features to distinguish between fibrous dysplasia and ossifying fibroma, while proportions of woven bone trabeculae type is a poor distinguishing feature. This is the first demonstration of employing mixed bone trabeculae proportions   to distinguish between fibrous dysplasia and ossifying fibroma.




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