Masson’s Trichrome and AgNOR Study of Fibrous Dysplasia and Ossifying Fibroma in Lagos University Teaching Hospital Patients
Olujide Soyele, Olajumoke Effiom and Onatolu Odukoya
World Journal of Pathology 2013, 2:16
Fibrous dysplasia (FD) and ossifying fibroma(OF) pose diagnostic challenges.This study attempts to differentiate between the two lesions, using AgNOR and Masson’s Trichrome staining techniques.
A retrospective study on FD and OF.
Materials and methods
Forty cases each of fibrous dysplasia and ossifying fibroma were randomly selected from the oral biopsy archives of Lagos University Teaching Hospital. Three 5 microns paraffin sections per case were stained with Hematoxylin and Eosin, Masson’s Trichrome and AgNOR respectively. Proportions of bone trabeculae type and mean AgNOR scores for both lesions were analyzed for χ² and t statistics respectively using the Epi-info statistical package.
Proportions of Lamella bone trabeculae type in ossifying fibroma(52.5%) was significantly higher than in fibrous dysplasia (30.0%); proportions of mixed bone trabeculae type in fibrous dysplasia (70.0%) was significantly higher than in ossifying fibroma (50.0%) (p< 0.05). Proportions of woven bone trabeculae type in fibrous dysplasia (60.0%) was not significantly higher than in ossifying fibroma (55.0%) (p> 0.05). Mean AgNOR score for ossifying fibroma (130.4 ± 32.3/ 100 cells) was significantly higher than for fibrous dysplasia (92.3 ± 27.4/ 100 cells) (p < 0.05).
Proportions of Lamella and mixed bone trabeculae types and mean AgNOR scores are important features to distinguish between fibrous dysplasia and ossifying fibroma, while proportions of woven bone trabeculae type is a poor distinguishing feature. This is the first demonstration of employing mixed bone trabeculae proportions to distinguish between fibrous dysplasia and ossifying fibroma.