World Journal of Pathology Volume No 6

Original Article Open Access

Molecular Analysis of X-Linked Inhibitor of Apoptotic Protein (XIAP) and Caspase -3 in Oral Cancer

Manisha Shrivastava, Rashmi Bathri, Shweta Mishra, Pallavi Joshi, Anupriya Rathore and Manoj Pandey
World Journal of Pathology 2017, 6:9

Abstract

Introduction

Over-expression of anti-apoptotic proteins such as X-linked inhibitor of apoptotic protein (XIAP) leads to defects in caspase-3 mediated apoptosis regulation and is one of the main causes of cancer development and is associated with poor clinical outcome. Targeting of XIAP in conventional cancer therapy has the potency to significantly improve success rates of cancer treatment and the survival of patients. Our study is a preliminary effort to study the gene expression of less studied XIAP and caspase -3 in oral cancers by amplification of these genes through PCR method and 8-oxodG, a marker of oxidative damage.

Material and Methods

This Prospective pilot study is based upon the expression of XIAP and caspase protein among patients with oral cancer. PCR- gel electrophoresis was performed to analyze the gene expression and level 8-oxo dG was measured by performing ELISA.

Results

Out of the 11 samples, 7 pure DNA samples showed amplification of caspase-3 and β-Actin gene as internal control. The median value of 8-oxodG was 9.47 nM (interquartile range: 5.64–41.13 nmol).

Conclusions

We have optimized the protocol for the molecular analysis of these genes through PCR and propose to continue the study on a larger sample size with suitable controls and also include real time PCR and Western blot techniques for evaluating the expression of XIAP protein and caspase -3 and its role in future target therapy in oral cancer patients.

Keywords

Apoptosis; oral cancer; XIAP; caspase-3; oxidative stress.




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