The Effect of Lagenaria Siceraria (Molina) on Acute Lung Injury Induced by Oleic Acid in Rats
Fahri Yetişir, Ebru Salman, Özkan Önal, Dilara Zeybek, Mustafa Aksoy, Ahmet Dostbil, Halit Yetişir, Figen Kaymaz, Süheyla Ünver and Mehmet Kılıç
World Journal of Surgical Research 2013, 2:8
Oleic acid has been used to induce acute lung injury in experimental studies. Lagenaria siceraria (Molina) has been reported to have cardiotonic, hepatoprotective, immunomodulatory, antihyperglycemic, antihyperlipidemic, analgesic and anti-inflammatory properties. In this study, we investigated the effects of Lagenaria siceraria (LS) fruit juice on acute lung injury induced by oleic acid in rats.
Material and Method
Thirty five adult female Sprague Dawley rats divided into 5 groups, 7 in each. Group I and group II received normal saline for 30 days, group III, IV and group V received LS at a dose of 200 mg/kg, 200 mg/kg and 400 mg/kg for 30 days by gavage respectively. 100mg/kg oleic acid was administered i.v in group II, group IV and group V. Histopathological examination of the lung was performed with light and electron microscopy. Levels of protein carbonyl, malondialdehyde, superoxide dismutase, catalase and glutathione peroxidase levels were measured in tissue samples. Levels of TNF alpha, IL10, IL6 and total tissue oxidant status and total tissue antioxidant status were measured in serum samples.
Light microscopy showed that LS at both doses decreased the total lung injury score compared to group II. In electron microscopy, LS at both doses decreased the thickness of the fused basal lamina when compared to group II. TNFα, IL6 in serum and MDA in tissue were higher in group II when compared to group me and attenuated in group V significantly.
Lagenaria siceraria attenuated the extent of acute lung injury induced by oleic acid in rats.
Lagenaria Siceraria, lung injury, oleic acid, pneumocyte.