Dexmedetomidine Pretreatment Attenuates Mesenteric Ischemia Reperfusion Injury In Rats
Ebru salman, Fahri Yetişir, Özkan Önal, Dilara Zeybek, Öztuğ Önal, Banu Yürekli, Ismail Yürekli, Ayşegül Süzer and Mehmet Kılıç
World Journal of Surgical Research 2013, 2:7
To investigate the effect of dexmedetomidine pretreatment in a rat model of mesenteric ischemia-reperfusion injury using biochemical markers and histopathological methods.
Material and Methods
A total of 28 female Sprague Dawley rats weighing between 230-300 gr were randomly divided in to 4 groups, 7 rats in each. Group I in which sham surgical preparation including isolation of SMA without occlusion was performed. Group II in which intestinal I/R was produced by clamping SMA for 1 hour and declamping for 3 hours, group III sham operated dexmedetomidine received dexmedetomidine at a dose of 25 mcg/kg i.p. Group IV dexmedetomidine was given at a dose of 25 mcg/kg i.p 30 min before intestinal ischemia induced. Rats were sacrificed at the end of the reperfusion period. Malondialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), catalase, and glutathione peroxide (GPX) levels were analyzed in intestinal tissue samples. Tissue total antioxidant status (TAS), tissue total oxidant status (TOS), TNF alpha, IL6, IL10 values were measured from serum samples 3 hours after reperfusion. The histopathological examination scores were determined using the intestinal tissues.
The mean TOS, TAS, GPx, SOD, catalase, MDA and IL10 values were not significantly different between group II and group IV. There were significant difference in the mean PC, TNF alpha and IL6 values between group II and group IV. The histopathological examination scores of intestinal tissues were significantly higher in group II compared to group IV (P<0.05).
Pretreatment with dexmedetomidine attenuates intestinal ischemia-reperfusion injury in rats. Dexmedetomine prevents remarkable morphological alterations in intestinal tissue and attenuates proinflammatory cytokines and protein oxidation.
Dexmedetomidine, ischemia reperfusion, protein carbonyl, TNFα.